Coding

Part:BBa_K3063111:Design

Designed by: Ng Tsz Chun   Group: iGEM19_Hong_Kong_HKU   (2019-09-13)


FlhDC transcriptions factors (Salmonella Typhimurium) with lac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lac suppression is absence in Salmonella Typhimurium, and wild type Lac promoter would lead to constitutive expression of genes in Salmonella. A strong constitutive promoter and terminator is used.


Source

FlhDC gene comes from genome of Salmonella Typhimurium.

References